mRNA Expression of Genes Involves In Intrinsic Apoptotic Pathway During HIV Infection and Therapy and Its Relation In Circulating cd4 T-Lymphocytes

Background: CD4 T-lymphocyte apoptosis in Human Immunodeficiency Virus infection may occur due to activation of the intrinsic apoptotic pathway or triggered via death receptors. In this study, differential expression genes involved was investigated on circulating T-lymphocytes HIV-infected patients and healthy controls. Methods: The study included 55 HIV who were (1) asymptomatic antiretroviral therapy naive, (2) symptomatic (3) therapy. As control 20 persons included. isolated from blood samples controls using density gradient centrifugation method by negative selection peripheral mononuclear cells (using CD4+ T Cell Isolation Kit followed total RNA extraction cDNA synthesis. Genes including BAX, BAK, BIM, MCL-1 BCL-2, Caspase-3, Caspase-9 Calpain-1 selected analyzed real-time PCR detect their expression. fold changes gene PLHIV groups compared with that Results: Among under no significant upregulation downregulation observed exception BCL-2 which statistically (p <0.05). None studied showed any correlations between expressions count. Conclusion: Gene not have effect count treatment. Bangladesh J Medicine 2022; 33: 176-185